Non-heme protein in the giant extracellular hemoglobin of the earthworm Lumbricus terrestris.
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Non-heme protein in the giant extracellular hemoglobin of the earthworm Lumbricus terrestris.
The protein/heme mass ratio for the extracellular hemoglobin of the earthworm Lumbricus terrestris has been redetermined. We find a value of 19,000 g of protein per mol of heme. Four major, heme-containing chains (a, b, c, and d), present in equal proportions, have a total molecular mass, with four hemes, of 69,664 Da based on their sequences. The intact hemoglobin comprises 12 subunits that fo...
متن کاملStoichiometry of subunits and heme content of hemoglobin from the earthworm Lumbricus terrestris.
The extracellular hemoglobin (Hb) of the earthworm, Lumbricus terrestris, has four major O2-binding chains, a, b, c (forming a disulfide-linked trimer), and d ("monomer"). Additional structural chains, "linkers," are required for the assembly of the approximately 200-polypeptide molecule. The proportion of linker chains had been reported to be one-third of the total mass on the basis of densito...
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The complete dissociation of the hexagonal bilayer structure of Lumbricus terrestris hemoglobin (3900 kDa) at neutral pH, in the presence of urea, guanidine hydrochloride, sodium perchlorate, potassium thiocyanate, sodium phosphotungstate, and sodium phosphomolybdate, followed by gel filtration at neutral pH on Sephacryl S-200 or Superose 6, produced two fragments, II (65 kDa) and III (17 kDa);...
متن کاملThe extracellular hemoglobin of the earthworm, Lumbricus terrestris. Determination of subunit stoichiometry.
The giant extracellular hemoglobin of the earthworm, Lumbricus terrestris, has four major O2-binding chains, a, b, and c (forming a disulfide-linked trimer) and d ("monomer"). Participation of additional "linker" chains L1, L2, and L3 is necessary for the assembly of the approximately 3,900+ kDa two-tiered hexagonal structure. We have determined the proportions of linker chains, trimer, and cha...
متن کاملCeramide glycanase from the earthworm, Lumbricus terrestris.
Ceramide glycanase (CGase) is an enzyme that cleaves the linkage between the sugar chain and the ceramide. To make this enzyme readily available, we have developed a simple method for preparing it from the earthworm, Lumbricus terrestris. The method involves Bio-Gel A-0.5m, octyl-Sepharose and p-aminophenylthiogalactoside-agarose column chromatography. By gel filtration, the molecular mass of e...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1988
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.85.24.9461